1/16/2024 0 Comments Lloyds tsb advert people![]() ![]() “We still have work to do – absolutely, as do all of the banks that have created mistrust since the banking crisis – but we are happy to do that work. We’ve done a lot internally to pay back what we owe to the government and our body language in the market is to show we’re here for customers. The Lloyd’s Lab is the heart of innovation for Insurance. Our marketing is all about building trust again. Reddan admits the bank still has a long way to go in fully rebuilding trust. It declinded 2.1 points to -2.3, putting Lloyds at 27 th on the table, with only Barclays, HSBC and the Royal Bank of Scotland worse off. However, Lloyds’ buzz ranking, a balance between positive and negative consumer sentiment of a brand, has fallen at a statistically significant rate over the last 12 months. ![]() Lloyds’ Index score is up 2.4 points to give it a score of 0.5 – placing it 15 th on a list of the UK’s 30 biggest banking brands. Last month, it reported a 7% fall in annual profits as it blamed £2.1bn of additional charges it had set aside to cover the ongoing payment protection insurance (PPI) scandal.Īnd according to YouGov BrandIndex, its Index ranking, which is measures consumer perception of metrics including quality, value and reputation, has risen by a statistically significant rate over the last year. Lloyds, much like its rivals in the banking sector, is no stranger to controversy following the global financial crisis. Why would you be ashamed of fully utilising an iconic British brand?” Turning the corner Bringing the horse back shows we are proud of our heritage. When questioned if bringing back the black horse could appear like the banking brand was ‘playing it safe’, Reddan countered: “It isn’t too safe, I disagree. People reacted positively to it and realised that our history creates a big element of trust,” she added. “It beat all our metrics and achieved great cut through. Reddan, without revealing numbers, claimed the campaign was a “huge success”. That isn’t genuine.” ‘Utilising our iconic British brand’įollowing a break, Lloyds brought back the black horse last June to celebrate its 250 th anniversary in a campaign that spanned historic events such as World War 1. Our marketing isn’t people jumping up and down dancing because they’ve opened a current account. ![]() “The temptation is too often to sugar coat real life. We are on the public’s side whatever it is and that’s a brave stance for a bank to take. We understand there are good things and bad things in the world. “What stands out about this new advert is it isn’t sugar coated. We want to reintroduce the strength of the horse to rebuild trust. It’s been with us for 250 years and is crucial to our heritage. She told Marketing Week: “In our testing, spontaneous association of the black horse was at 94% so as a brand icon it really is comparable to the Apple logo or the Nike tick. Lloyds initially brought back the black horse in last year’s 250 th anniversary campaign and Reddan says it is here to stay. ![]() Activity will run in TV, print, digital, cinema, outdoor and social media. In recent years, many businesses have produced pro-LGBT ad campaigns – but such ads are not always put into heavy circulation, often resorting to attracting attention online.The new campaign, the second since Lloyds switched its account to adam&eveDDB last year, features the Lloyds black horse dramatically galloping through real-life scenarios including the birth of a child and a funeral. Lloyds Banking Group – proud sponsors of last year’s PinkNews Awards – has a strong history of supporting LGBT rights. “We’ve adopted a contemporary and distinctive approach, focusing on the diverse and sometimes challenging moments in the lives of our customers today.” Lloyds marketing director Catherine Kehoe explained: “The new campaign builds on the phenomenal success of the 250th anniversary campaign, showing how we’ll always be by our customers’ side, giving them the financial confidence to take their next step. One such still ad features the same-sex couple with the words: “He said yes”. Lloyds – which was named as the top bank in the Stonewall Index and the top LGBT employer in Scotland and Wales earlier this year – intends to go big with the new campaign, with ads also set to go out across cinemas, print ads and outdoor billboards. The clip also prominently features a same-sex couple in the midst of a proposal, in the middle of a crowded street – who are also shown embracing towards the end. It features people frozen in various stages of life – including a new mother, a man dropping his daughter off at school, and an elderly woman attending a funeral – as a horse representing the bank rides past. The prominent new ad campaign, which was first aired by the banking giant on Friday, is titled ‘For Your Next Step’. ![]()
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1/16/2024 0 Comments African shoebill storks![]() ![]() The birds ensure that they protect and defend their territories from enemies, intruders and other Shoebills. Their nests are sparsely distributed and the distance between one nest to another can be between 2 to 4 square Kilometers. It is rare to find them in a large group. Their wings make about 150 flaps per minute compared to the average bird that make 70 wingbeats per second which sums up to 4000 flaps per minute.Ī Solitary Bird: Shoebill Storks are very solitary birds. It should be noted that for most of their time, shoebills are dead silent.Ī slow bird: The Shoebill Stork is one of the slowest flying birds. The sound of their clutters are similar to that of a machine gun. In most cases, it is the older and stronger chic that survives after doing away with the weaker ones.Ĭlutters that sound like a Gun: Shoebill Storks clutter their bills loudly to express some sort of excitement especially during courtship and or when communicating with others of their kind. The strongest chic does this through bullying or making it uncomfortable for their weaker siblings to continue living within the nest. This is the killing of their weak and younger siblings when the adults go out hunting. The older chicks tend to practice siblicide. Only one chic survives because of siblicide: Whereas adult Shoebill Stork are great parents, their efforts are sabotaged by their own chicks. It usually takes about a month for the incubated eggs to hatch into chicks. Shoebill Stork cool their eggs with water fetched using their large beaks. ![]() The Shoebill Stork will ensure that the eggs are well incubated and that no predators can access them. Their nests are built on floating vegetation. The eggs are few (2 to 3) but relatively safe because both parents put in effort to look after the eggs from their nest. Great Parents: The female Shoebill Stork lays its eggs at the end of the rainy season. Once they reach maturity, a male and female will pair up to start breeding. An adult bird is considered to be mature when they are 4 years. A mature Shoebill Stork weighs about 4 to 7 kilograms. It is estimated that the Shoebill Stork can fly at a top speed of 35 to 48 kilometers per hour using its broad wings that measure between 7.5 to 8.5 feet. The Shoebill Stork has a long life span of about 35 years while living in the wild and about 50 years in captivity. Their diet also includes snails, water snakes, lizards, rodents and sometimes baby crocodiles. The Shoebill Stork often beheads its prey before eating it using its razor sharp beak.įeeds on mainly fish and has a long lifespan: The Shoebill Stork primarily feeds on fish like tilapia, cat fish and its favorite the lung fish. ![]() When a Shoebill Stork knows that hippos are around, it positions itself and gets ready to strike on the disturbed fish. As hippos move around a swamp, they create pathways and disorganize fish hiding in the mud. The Shoebill Stork also takes advantage of the commotion left behind by large animals like hippos. Standing still, it positions itself around such pools waiting for the nearly suffocating fish to come for oxygen in the surface and then launch a surprise strike. The Shoebill Stork also takes advantage of water pools that are poorly oxygenated to ambush its prey. Shoebill Storks has a tendency to stand still and motionless on floating vegetation for long periods of time just to appear harmless before catching their prey by surprise. It will carefully calculate it’s moves before striking prey. The Shoebill Stork’s beak measures up to 20cm in width and 24cm in length enabling it to hunt both small and large prey.Īn Excellent Hunter: The Shoebill Stork is a very tactful day and night hunter. The title of longest beak belongs to the Toucan. One of the Largest beaks in the world: The Shoebill Stork has the 3rd longest beak in the world. Here are other interesting facts about the Shoebill Stork that you might not know: – Facts About the Shoebill Stork Pelicans and herons also belong to this group and thus have a similar genetic composition. They found that the bird actually belongs to the pelecaniformes and Balaenicipitidae family. The Shoebill Stork was at first considered to belong to the stork family but Researchers later discovered something different. ![]() 1/16/2024 0 Comments Capital city of nova scotia canada![]() ![]() It’s a quintessential red-topped lighthouse standing on a rocky shore overlooking the Atlantic. ![]() Once you see it, you’ll understand why it’s one of the most photographed lighthouses in the world. There are some 170 lighthouses in Nova Scotia, but Peggy’s Cove Lighthouse is the most famous. The city has a youthful, arty vibe and is worth visiting for a couple of days. Take the ferry over to Dartmouth across the harbor, known as ‘Halifax’s Brooklyn’ and check out the live music at New Scotland Brewing Company. Stroll the waterfront boardwalk, grab a lobster roll, and spend the evening at a local pub. It’s home to half a dozen universities so it has a lively nightlife, a thriving music scene, and countless trendy restaurants and craft breweries. Halifax is Nova Scotia’s cool capital city. Take a tour of the Halifax brewery to learn more and sample some of the limited edition beers at “Stag’s Head” pub at the end of the tour. Today, the 200-year-old brewery is one of the oldest in North America. He opened his brewery in 1820, became mayor of Halifax, and was so wildly popular that Halifax throws a massive birthday party for him on the waterfront every October. Tour the Alexander Keith’s BreweryĪlexander Keith is a legend in Nova Scotia. The most popular time to visit is July, August, and September but many people come to see the autumn leaves change in October. Make sure to bring your own water, good shoes, and clothing layers as the weather can change quickly. The hike is suitable for all levels and takes between 1.5-3 hours. It’s a breathtaking walk on which you might even encounter moose. It stretches 6.5 kilometers (4 miles) through thick forest and then along the coast to reach a viewing platform overlooking the ocean. The Skyline Trail is easily the most popular hike in Cape Breton Highlands National Park. Top 5 Things to See and Do in Nova Scotia This travel guide to Nova Scotia can help you plan your trip, save money, and make the most of your visit to this beautiful east coast province! In short, Nova Scotia is a province perfect for road trips.Īnother bonus: Nova Scotia doesn’t see nearly as many tourists as the country’s larger cities, making it a somewhat off-the-beaten-trail destination that’s much more affordable than many of the more popular cities in Canada. Drive further north, and you’ll hit scenic Cape Breton Island which comes alive with vivid fall foliage each year along its Cabot Trail. Outside the capital city of Halifax, Nova Scotia is dotted with tiny fishing villages and coastal towns. That welcoming atmosphere - combined with over 100 beaches, picturesque lighthouses, fresh seafood, and endless rugged coastline - makes visiting Nova Scotia an exciting (and underrated) destination in Eastern Canada. They might just be right because you’ll feel welcome the minute you step foot in this gorgeous Maritime province. Nova Scotians like to boast that their province is the friendliest place in Canada. ![]() ![]() ![]() It has a removable cover for easy washing, and if your want to get a 400 thread count, 100% egyptian cotton cover, you can get it as well. You can use it in two different postings. There are three sizes available with different inclinations, 30 degree, 20 degree, and 15 degree approx. Generally, memory foam pillows get hot and sweaty, but this InteVision foam bed wedge pillow has a patented air flow technology that keep you cool while providing support and comfort. On it, there is a layer of memory foam which is softer and provides comfort by taking shape of your back. The inner core is made of a firm foam that provides support. Used and loved by thousands of people, it is a high quality, well built, bed-wedge-pillow that supports your back while reading in bed. Memory foam wedge in 3 different inclinations for back support while sitting up or lying down. ![]() The quality of the memory foam is great and it will retain its shape over time. Your can separately buy a pillow case for it, as linked above. This will work for you.Ĭleaning is easy, just remove the cover and do a machine wash. Its size is perfect for almost all, whether you are short or tall. ![]() I have listed other pillows with either better air circulation technology, or with gel infused memory foam that solve this problem.Įither get one with better air flow, or place a thin pillow in between your back and the pillow. Memory foam takes shape of your back which enhances support, but it also causes overheating and in some cases sweating, which is not a great experience. Providing back support while being comfortable and having some give to it. Xtreme Comforts simple is for you, If you are looking for a wedge pillow that is entirely made of memory foam. Memory foam wedge in 3 different inclinations, and bamboo cover for back support while reading in bed ![]() 1/14/2024 0 Comments Craftsman 12 volt battery![]() ![]() I’ve driven 3 1/2″ drywall screws into plywood and pine 2×4’s without pilot holes and the drill had more than enough power. I was actually surprised at how much power this 12 volt drill has (+2). Inside view of the case The case zipper tends to come apart, which is annoying.Įven though the drill uses only 12 volts, it packs a punch! There is plenty of power for most household projects and can be used for the occasional larger project. Because of this “feature”, if you unzip the lid almost all the way, the end of the zipper will pull apart (see photo). I have no idea why you would want to do this. You can unzip the top and completely remove the lid. Overall, I like the case with 1 exception. It is small so, it is easy to carry around, but has enough room to store the drill, 2 batteries, and the charger or a box of bits (+1). The case is a semi-rigid, soft-sided case. The speed selector is definitely the biggest flaw of this otherwise excellent drill (-2). It is not always obvious when the speed selector is between speeds without pulling the trigger. When the slide gets stuck between speeds, the drill makes a horrible noise and won’t spin. The selector is plastic and slides forward for “Lo” and backward for “Hi” but doesn’t always slide all the way. On top of the drill there is a 2 position speed selector which allows you to select “Hi” speed and “Lo” speed. The light looks bright but is pretty much useless. In general, I think lights on drills are always useless. The light is pretty much useless unless you are drilling in almost complete darkness in which case you should just get a flashlight (-+0). There is an LED light that comes on when you press the trigger. The drill has a 17 position clutch that seems to work well but I don’t use it much (-+0). My old drill had a 3/8″ chuck and I have never needed a larger chuck (-+0) but if you need a 1/2″ chuck then look elsewhere. The drill has a 3/8″ chuck while many newer drill have a 1/2″ chuck. The 20oz bottle-sized drill can stand upright on a flat surface. The shape of the drill allows it to stand upright on a flat surface, it isn’t very stable like this, but it is handy nonetheless (+1). The batteries are much smaller and lighter than my old 18volt NiCd’s (+2). My initial reaction was that the grip was too thick (-1), and I have average to above average sized male hands, but I’ve gotten used to it. The drill itself is compact and light weight but feels very solid and durable in your hand (+1). ![]() The drill was within my price range (+1) and I’ve always had good experiences with Craftsman products (+1). You may be able to find other online merchants. The only place you can buy Craftsman tools is at Sears because Sears owns the Craftsman brand. The drill comes with 2 lithium-ion batteries, a charger, a case, and screwdriver bit and costs about $100. Craftsman NEXTEC 12Volt lithium-ion drill combo I liked the compact size and weight of lithium-ion drills but was put off a little by their higher price tags. My old drill was large and heavy and not very easy to carry around or to pull out to drill one quick hole so, I was interested in slightly smaller drills but I also looked at larger models. My old 18volt drill had plenty of power for me so I looked mostly at models in the 14-18 volt range. I wanted a drill with enough power for typical household repairs and projects, one with good batteries, one that was easy to use, and one that cost about $100-125. This review is my personal opinion on this product. I have not received any form of compensation from Sears or Craftsman. ![]() What follows is my review of that drill after using it for several months. I did a TON of homework, checked out a bunch of different drills, and finally decided on the Craftsman NEXTEC 12Volt lithium-ion drill ( Amazon link). I figured out that a new drill would cost about the same as 2 new batteries so, I went shopping for a new cordless drill. The NiCd batteries for my old Craftsman 18Volt cordless drill finally died and I have been unable to fix them (yet). ![]() 1/14/2024 0 Comments Parikia paros hotels![]() ![]() Show more Summary of reviews Sunset View Hotel is a highly recommended resort-style hotel located on a hill overlooking Parikia bay. Overall, Paros Palace guarantees a relaxing and indulgent vacation experience. ![]() The beds are firm and comfortable and the private pools available to guests are perfect for a luxurious and relaxing getaway. The pool area is amazing with stunning views of the sunset and guests can relax in the large resort pool or enjoy a personal pool in their suite. The staff are exceptionally kind, helpful and professional. The hotel is spotlessly clean and the staff go above and beyond to ensure guests have a wonderful stay. The rooms are spacious, beautifully designed with Cycladic decor and equipped with all necessary amenities. The breakfast buffet is fantastic with both sweet and savory options available and the quality and freshness of the food is commended. The staff are lovely and offer shuttle services to the town and the beach. The property is beautiful with spectacular views over the bay of Parikia. The hotel is well located, making it ideal for those who want to enjoy some quiet but not be too far from the city center. Show more Summary of reviews Paros Palace is a stunning hotel with an amazing location that offers great sunset views and is close to the main town. Overall, Pyrgaki Hotel is a great value for the price and a perfect way to unwind after a day of exploring the local area. While some guests felt that the hotel did not match their expectations, others enjoyed the variety and quality of the breakfast served on a beautiful terrace with views of the sea and mountains. The Pyrgaki Hotel is a great option for families looking for a relaxing and personalized vacation experience. The outdoor pool is stunning and offers plenty of comfy seating and loungers, making it the ideal spot for relaxing in the sun or enjoying the amazing view of the harbor. The staff is super friendly, helpful and accommodating, treating guests like family and going above and beyond to ensure their experience is memorable. The rooms are comfortable and spacious with excellent views of either the sea or Parikia and the hotel is exceptionally clean with daily room cleaning and maintenance. The breakfast is generous and varied with a great selection of both continental and cooked items, served on a large and beautiful terrace with excellent sea views. The hotel is situated in a lovely location with stunning views of the Parikia Bay and is in a quiet and peaceful area only a 20-minute walk away from the town. Show more Summary of reviews Pyrgaki Hotel is a great choice for those seeking a tranquil and picturesque escape. ![]() 1/14/2024 0 Comments Octonauts dwarf lantern shark![]() ![]() Spookfish – “The Scary Spookfish” (Season 1, episode 23) Lemon shark – “Lost Lemon Shark” (Season 1, episode 22)ĭolphin – “The Baby Dolphin” (Season 1, episode 23) Seahorse – “The Seahorse Tale” (Season 1, episode 21)Īnemone – “The Enemy Anemone” (Season 1, episode 21)Ĭuttlefish – “Crafty Cuttlefish” (Season 1, episode 22) Worm – “The Midnight Zone” (Season 1, episode 20)īlue whale – “The Mixed Up Whales” (Season 1, episode 20) Parrotfish – “Pirate Parrot Fish” (Season 1, episode 19Įlectric torpedo ray – “Electric Torpedo Rays” (Season 1, episode 19) Sardine – “The Sardine” (Season 1, episode 17)Įel – “The Eel Ordeal” (Season 1, episode 18)ĭolphin, coral reef- “The Dolphin Reef Rescue” (Season 1, episode 18) Kelp – “The Kelp Forest Rescue” (Season 1, episode 16)Įlephant seal – “The Enormous Elephant Seal” (Season 1, episode 17) Snapping shrimp – “The Snapping Shrimp” (Season 1, episode 16) Sea star – “The Lost Sea Star” (Season 1, episode 15)Ĭomb jelly- “The Giant Jelly” (Season 1, episode 15) Orca – “The Arctic Orcas” (Season 1, episode 14) Slime eel- “The Slime eels” (Season 1, episode 14) Leatherback sea turtle – “The Giant Whirlpool” (Season 1, episode 13) Snot sea cucumber – “The Snot Sea Cucumber” (Season 1, episode 13) Pilot fish, whitetip shark – “The Hungry Pilot Fish” (Season 1, episode 12)īeluga whale – “The Beluga Whales” (Season 1, episode 12) Hermit crab – “The Hermit Crab” (Season 1, episode 11) Kelp – “The Giant Kelp Forest” (Season 1, episode 11) Spider crab – “Giant Spider Crab” (Season 1, episode 10) Humuhumu fish – “Humuhumus” (Season 1, episode 10) Vampire squid – “The Vampire Squid” (Season 1, episode 9) ![]() Sailfish- “The Speedy Sailfish” (Season 1, episode 9) Jellyfish – “The Jellyfish Bloom” (Season 1, episode 8) Whale shark – “The Whale Shark” (Season 1, episode 7)Ĭookiecutter sharks – “The Cookiecutter Shark” (Season 1, episode 8) ![]() Orca whale, fiddler crabs – “The Orcas” (Season 1, episode 6)īlobfish – “The Blobfish Brothers” (Season 1, episode 6)ĭecorator crab – “The Decorator Crab” (Season 1, episode 7) Remipedes – “The Remipedes” (Season 1, episode 5) Urchin, carrier crab – “The Crab & Urchin” (Season 1, episode 4)Īlbino humpback whale – “The Albino Humpback Whale” (Season 1, episode 5) Octopus – “The Monster Map” (Season 1, episode 4) Narwhal – “The Narwhal” (Season 1, episode 3) Walrus – “The Walrus Disguise” (Season 1, episode 2)įlying fish – “The Flying Fish” (Season 1, episode 3) Giant squid – “The Giant Squid (Season 1, episode 1)Ĭrustaceans, algae – “The Great Algae Escape” (Season 1, episode 2) Reef lobster – “The Undersea Storm” (Season 1, episode 1) Shellington, Peso, Tweak, Sauci, and Tunip work together serving as the “octonauts” – eight navigators at the sea.Īfter episodic creatures come in providing a plot based on their unique species adaptations, each half of the show ends with a Creature Report – a song explaining how the creature works. There’s a team element to their science investigations, as Captain Barnacles, Lt. Each episodes is usually broken into two parts, with both halves highlighting a new ocean animal. In English.Ĭookiecutter sharks - Lost lemon shark - Dwarf lantern shark - Giant jelly - Hungry pilot fish - Porcupine puffer.Ĭookiecutter sharks: Captain Barnacles and the crew investigate why mysterious holes have started to appear in their equipment - Lost lemon shark: The underwater explorers go in search of a lemon shark that has hurt its nose and is struggling to find its way home - Dwarf lantern shark: Captain Barnacles and the team try to find an injured dwarf lantern shark - Giant jelly: The crew tries to rescue Peso when he becomes trapped inside a giant jelly - Hungry pilot fish: A lost pilot fish who eats ocean gunk cleans up the crew's dirty gups, while Captain Barnacles tries to work out why a whitetip shark is chasing the gup-a - Porcupine puffer: Barnacles, Kwazii and Peso venture back inside a whale shark to retrieve a porcupine puffer fish that was accidentally swallowed.Octonauts is a 23 minute TV show on Netflix. Special features: Learn more about marine life in 6 creature reports. Originally broadcast as episodes of the television program in 2010-2013. 1/14/2024 0 Comments Plants vs zombies books 11![]() ![]() OL20007370W Page_number_confidence 84.52 Pages 86 Ppi 300 Republisher_date 20190926125807 Republisher_operator Republisher_time 369 Scandate 20190921093235 Scanner Scanningcenter cebu Scribe3_search_catalog isbn Scribe3_search_id 9781616558321 Tts_version 3. Message the Moderators if you see anything that needs to be removed Posts of the "Repost with your favorite plant" category are banned.Access-restricted-item true Addeddate 08:59:07 Associated-names Chan, Ron, penciller, cover illustrator Rainwater, Matthew J., illustrator Dutro, Steve, letterer Bookplateleaf 0002 Boxid IA1665912 Camera Sony Alpha-A6300 (Control) Collection_set printdisabled External-identifier Zombies Heroes - Crafting System Guide (Made by /u/the_ninjadillo)īattleZ FAQ (Made by /u/Tigerol) Our Friends Ghost Pepper Manual (Made by /u/boynedmaster)Īll Glory to the Hypno-Shroom: A Hypno-Shroom Guide (Made by /u/Magnemania) Most of us here aren't sure how to solve some of the issues you may be having, so use the above link and look for the game you need help with! If it's an issue related to your progress being lost, make sure you give them your player ID which can be found on the title screen if it's PvZ 2 or by clicking the gear icon if it's Heroes. ![]() ![]() Join our Discord Server: Where to get PvZ games These are permitted but only in certain circumstances, as they don't promote any real discussion. Low quality content is up to mod discretion. Memes/Jokes are fine but only if they are themed to match the game, no unrelated memes with just an edited title. This is an English speaking subreddit all posts must be in English. Feel free to message the mods if you're unsure if your post is appropriate. Posts containing any kinds of graphic material, including suggestive fan art, will be removed. Zombies comic book by Dark Horse Comics This is issue 1 of the. Dave the Villager 11: An Unofficial Minecraft Book (The Legend of Dave the Villager) by Dave Villager. Lawnmageddon Issue 1 Zebra Gamer 418K subscribers 91K views 5 years ago Today we check out the official Plants vs. Please under no circumstances flood the sub with nonsense! Zombies: Plant Your Path Junior Novel from the world’s largest community of readers. Zombies Zomnibus Volume 1 Book 1 of 2: Plants Vs. Zombies (Series) Paul Tobin Author Christianne Gillenardo-Goudreau Illustrator (2020) Plants vs. Posting, linking or describing cheats or hacks for any of the PvZ games is strictly forbidden and will result in a permanent ban. 647 Hardcover 759 List: 10.99 FREE delivery Thu, Jun 8 on 25 of items shipped by Amazon Or fastest delivery Tue, Jun 6 More Buying Choices 1.48 (43 used & new offers) Kindle Best Price in 30 Days 299 Available instantly Ages: 8 - 12 years Plants vs. Zombies (Series) Paul Tobin Author Kieron Dwyer Illustrator (2020) Plants vs. If your post is not related to the game this subreddit is dedicated to, please post it elsewhere! 78 Hardcover 1299 List: 19.99 FREE delivery Mon, Jul 10 on 25 of items shipped by Amazon More Buying Choices 3.08 (41 used & new offers) Kindle 599 Print List Price: 19. Offending comments may be removed and warnings issued. Zombies Zomnibus Volume 1 Book 1 of 2: Plants Vs. We have a wide audience consisting of children, teens, and adults. DARK THEME NORMAL THEME Rules of the Lawn This subreddit is a hub for all of the games in the PVZ series. Zombies 2 Everything you needed to know about Power Mints Update Power Mints are your plants’ best friends on the lawn (besides you). Zombies is an exciting series of games by PopCap, spanning over many genres such as tower defense, third-person shooter, and a strategy card game. A New Vision for PvZ 3 Has Taken Root Read the latest update from Matt Townsend about the next PvZ game. ![]() 1/14/2024 0 Comments U boat watch u1001 price![]() ![]() He was to design a new watch for their pilots. U-Boat Watches U-Boat Watches over AU 4,000.00 All Auction Buy It Now 54 results 2 filters applied Brand Band Colour Dial Colour Model Bezel Colour Buying format Condition Delivery options All filters U-Boat 8529 Chimera automatic SS Limited Edition 46mm 10ATM AU 4,157.77 AU 45. All U-Boat watch straps are entirely handmade by expert craftsmen in Italy from selected leathers and materials. 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KRONOS 360 is a trusted third-party marketplace that secures watch transactions and ensures the expertise of every luxury watch, authentication, traceability, logistics and delivery with a 2-years warranty. 3,250.00 Free shipping U-Boat U1001 2280/1 Titanium Italian Diver 55mm Destro Limited Wrist Watch 2,800.00 + 17.10 shipping Hover to zoom Have one to sell Shop with confidence Authenticity Guarantee This item is verified by an authenticator before delivery. KRONOS 360 offers you brand new luxury watch, pre-owned luxury watch and collector luxury watch from 100 major watch brands. KRONOS 360 is a platform for buying and selling luxury watches since 2016. Kronos 360, the luxury watches marketplace About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators. ![]() 1/14/2024 0 Comments Southern blotting technique![]() ![]() The process is done by either electroblotting or capillary blotting. Although this step determines the name of this technique “Southern blotting,” the term is typically used to describe the entire procedure. DNA thus obtained are double-stranded, and is therefore denatured to single-stranded DNA by dipping the gel in an alkaline solution.īlotting refers to the transfer of the fragmented DNA sequence to the nitrocellulose membrane or nylon membrane. Acrylamide gels can alternatively be used for good resolution of smaller DNA fragments (<800 bp). (Image source: National Human Genome Research Institute) Extraction, purification, fragmentation, and separation of target DNAĭNA is extracted from the target source and is broken into small fragments using restriction endonuclease enzyme.įragmented DNA is then electrophoresed on an agarose gel to separate the fragments according to their molecular weights. ![]() DNA loading buffer, TBE Buffer for electrophoresis Steps involved in Southern blotting Southern blotting technique.Enzymes: restriction nucleases, RNase A.Cellulose nitrate or nitrocellulose membrane filter with uniform porosity.The probe also has a radioactive atom or a fluorescent dye label, that following hybridization, permits the DNA fragment of interest to be detected from different DNA fragments present on the membrane. The membrane is then treated with a small piece of DNA or RNA called a probe, which has a complementary sequence to the target DNA. ![]() Following separation, the double-stranded pieces of DNA are denatured into single strands within the gel and transferred from the gel onto a blotting membrane. The target DNA is broken into small fragments using restriction endonucleases and is separated by electrophoresis. Extraction, purification, fragmentation, and separation of target DNA. ![]() |
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